The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of putative ketosteroid isomerase (YP_750657.1) from Shewanella frigidimarina NCIMB 400 at 1.80 A resolution. To be published
    Site JCSG
    PDB Id 3bb9 Target Id 378123
    Molecular Characteristics
    Source Shewanella frigidimarina ncimb 400
    Alias Ids TPS1708,YP_750657.1, 3.10.450.50, 343309 Molecular Weight 16031.33 Da.
    Residues 147 Isoelectric Point 6.28
    Sequence msagqsslsfahgdethpieqkafigvdsaagnvvkqfhaalqmgneaivrqslaanvqiyeggkvers lteyanhhmladmaylkgltitpkehqititgdiaistsishaqgeykgksidsmtmetlvlikqadgr wkithvhws
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 6
    Resolution (Å) 1.80 Rfree 0.228
    Matthews' coefficent 1.69 Rfactor 0.175
    Waters 611 Solvent Content 27.16

    Ligand Information


    Google Scholar output for 3bb9
    1. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org

    Protein Summary

    Gene Sfri_1973 from Shewanella frigidimarina ncimb 400  encodes a putative ketosteroid isomerase (YP_750657.1). Its genomic neighborhood is enriched in genes encoding proteins involved in copper resistance.

    The 3bb9 structure is similar to several NTF2-like proteins. Residues conserved  in 3bb9 do not superpose well with similar or identical residues of other NTF2-like proteins, but only with: the BXE_B1374 protein (PDB code 2owp; Z=14), the association domain of Ca2+/calmodulin-dependent kinase II (PDB code 1hkx; Z=14) and the ketosteroid isomerase (PDB code 1oh0; Z=13). The similarity between the clefts in 3bb9 and the ketosteroid isomerase (1oh0) indicates that both proteins might have similar function.

    1. What is the function of the the association domain of Ca2+/calmodulin-dependent kinase II (PF08332) present in a single domain proteins (lacking the protein kinase domain)?
    2. Is the reaction mechanism similar to that proposed by Kim et al.?

    Ligand Summary





    No references found.

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