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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of Type I restriction enzyme StySJI M protein (NP_813429.1) from Bacteroides thetaiotaomicron VPI-5482 at 2.20 A resolution. To be Published
    Site JCSG
    PDB Id 2okc Target Id 363548
    Molecular Characteristics
    Source Bacteroides thetaiotaomicron vpi-5482
    Alias Ids TPS1471,NP_813429.1 Molecular Weight 49981.39 Da.
    Residues 444 Isoelectric Point 5.10
    Sequence matnssteqsltkkvwnlattlagqgigftdyitqltyllflkmdaenvemfgeesaiptgyqwadlia fdgldlvkqyeetlkllseldnligtiytkaqnkidkpvylkkvitmideeqwlimdgdvkgaiyesil ekngqdkksgagqyftprpliqamvdcinpqmgetvcdpacgtggflltaydymkgqsaskekrdflrd kalhgvdntplvvtlasmnlylhgigtdrspivcedslekepstlvdvilanppfgtrpagsvdinrpd fyvetknnqlnflqhmmlmlktggraavvlpdnvlfeagagetirkrllqdfnlhtilrlptgifyaqg vkanvlffskgqptkeiwfydyrtdikhtlatnklerhhlddfvscynnrveiydaennpqgrwrkypv deiiardktslditwikpggevddrslael
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 2
    Resolution (Å) 2.20 Rfree 0.232
    Matthews' coefficent 2.47 Rfactor 0.182
    Waters 348 Solvent Content 50.28

    Ligand Information


    Google Scholar output for 2okc
    1. The structure of M. EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein
    CK Kennaway, A Obarska-Kosinska - Nucleic acids , 2009 - Oxford Univ Press
    2. Functional analysis of MmeI from methanol utilizer Methylophilus methylotrophus, a subtype IIC restriction-modification enzyme related to type I enzymes
    J Nakonieczna, T Kaczorowski - Applied and , 2009 - Am Soc Microbiol
    3. The fragment structure of a putative HsdR subunit of a type I restriction enzyme from Vibrio vulnificus YJ016: implications for DNA restriction and translocation activity
    NT Uyen, SY Park, JW Choi, HJ Lee - Nucleic acids , 2009 - Oxford Univ Press
    4. Characterization and crystal structure of the type IIG restriction endonuclease RM. BpuSI
    BW Shen, D Xu, SH Chan, Y Zheng, Z Zhu - Nucleic acids , 2011 - Oxford Univ Press
    5. Structural and Functional Analysis of the Engineered Type I DNA Methyltransferase EcoR124I NT
    JE Taylor, P Callow, A Swiderska, GG Kneale - Journal of molecular biology, 2010 - Elsevier
    6. Crystallization and preliminary X-ray diffraction analysis of the HsdR subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016
    NT Uyen, K Nishi, SY Park, JW Choi - Section F: Structural , 2008 - scripts.iucr.org
    7. Expression, crystallization and preliminary X-ray diffraction analysis of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016
    HJ Lee, K Nishi, JM Song, JS Kim - Acta Crystallographica Section F: , 2009 - scripts.iucr.org

    Protein Summary

    Bacteroides thetaiotaomicron descr protein NP_813429.1 is closely homologous to E.coli Type I restriction enzyme EcoKI M protein (EC  (2ar0)  with ~30% sequence identity.  However, the  two  domains  forming  these proteins have different mutual orientations, RMSD in flexible (FATCAT) alignment is 1.8 A over 390 aminoacids as compared to 13.9 A over 340 aa in a rigid alignment.

    Ligand Summary





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