The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of the protein BH2851 (10175472) from Bacillus halodurans at 2.12 A resolution. To be published
    Site JCSG
    PDB Id 2gfg Target Id 359067
    Molecular Characteristics
    Source Bacillus halodurans c-125
    Alias Ids TPS1413,10175472, _0044.000266_, 289541 Molecular Weight 22050.82 Da.
    Residues 192 Isoelectric Point 5.84
    Sequence mgkeieierktlvsketfkrlisqlhigegdfklqrnhyfetddfqlkkqssalrirekeaiftftlkq phpaglletnqtlskqeaklalesahfpsgevmdalrdlsipisqlkhigtlstsraeisyeqgilcld hssylgiedyeiefegtseehatvtfqeilktfsisqvptenkiqrffskkekn
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 3
    Resolution (Å) 2.12 Rfree 0.274
    Matthews' coefficent 2.23 Rfactor 0.225
    Waters 259 Solvent Content 44.46

    Ligand Information


    Google Scholar output for 2gfg
    1. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org

    Protein Summary

    The gene BH2851 from Bacillus halodurans encodes the protein NP_243717.  The sequence alignment identifies the presence of a CYTH domain observed in the adenylate cyclase family (PF01928). SCOP classifies 2gfg inside the CYTH-like phosphatases superfamily, CYTH domain family.

    The structure of 2gfg is similar (Dali Z-scr=15) to that of adenylate cyclase class IV (AC4) from Yersinia pestis PDB:3ghx.  The Yersinia pestis AC4 structure shows the complex of the enzyme with its product, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE (cAMP).  cAMP is the product of ATP cyclization by AC4 EC: The Bacillus halodurans structure was co-crystallized with an unknown ligand, which is located in the binding pocket of cAMP. A second Dali hit is with yet another AC4 structure, PDB:2een from Pyrococcus Horikoshii. It is annotated by the authors of the structure  as hypothetical protein PH1819. A promising candidate for comparison is given by PDB:3n10 an adenylate cyclase IV released on May 14, 2010. It includes cAMP and shares 139 equivalent residues with an rmsd of 2.56Å and 19% sequence identity using TopMatch for structure comparison. It is very plausible that 2gfg represents another AC4 homologue.  The AC4 has a distinct fold from that of AC classes II and III  [Ref].  

    cAMP is an important signaling molecule in virtually all prokaryotic and eukaryotic cells.  In prokaryotes cAMP is a catabolite derepression signal used to trigger the expression of metabolic pathways such as the lactose operon that are otherwise idle when glucose is plentiful.

    Ligand Summary





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