The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of 16S rRNA processing protein from Pseudomonas aeruginosa at 2.46 A resolution. To be published
    Site JCSG
    PDB Id 2f1l Target Id 357814
    Molecular Characteristics
    Source Pseudomonas aeruginosa pao1
    Alias Ids TPS1379,NP_252433.1, 289764 Molecular Weight 19788.51 Da.
    Residues 175 Isoelectric Point 4.86
    Sequence mptpaddlvvigkivsvygirgevkvysftdpldnlldyrrwtlrrdgeirqaelvrgrlhgkvlaakl kglddreeartftgyeiciprselpsleegeyywhqleglkvidqgrqllgvidhlletgandvmvvkp cagslddrerllpytgqcvlsidlaagemrvdwdadf
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 2.46 Rfree 0.237
    Matthews' coefficent 3.41 Rfactor 0.175
    Waters 44 Solvent Content 63.68

    Ligand Information


    Google Scholar output for 2f1l
    1. Cradle-loop barrels and the concept of metafolds in protein classification by natural descent
    V Alva, KK Koretke, M Coles, AN Lupas - Current opinion in structural , 2008 - Elsevier
    2. Structural characterization of the ribosome maturation protein, RimM
    S Suzuki, A Tatsuguchi, E Matsumoto - Journal of , 2007 - Am Soc Microbiol

    Protein Summary

    The gene PA3744 from Pseudomonas aeruginosa encodes the NP_252433 protein with two domains: a putative 16S rRNA processing protein belonging to the RimM (N-terminal domain) group (PF01782); and a PRC barrel C-terminal domain found in the RNA metabolism proteins of the RimM group (PF05239). STRING analysis indicates a functional link (score 0.99) of PA3744 with its genomic neighbor trmD, a tRNA-methyltransferase.

    The 2f1l N-terminal domain structure adopts a reductase/isomerase/elongation factor comon domain fold, inside the RimM N-terminal domain family; the C-terminal domain shows a PRC barrel domain fold, inside the RimM C-terminal domain family. The structures of homologous proteins  from other organisms have been determined: 3H9N (Dali Zscr=14), from Haemophilus influenzae; 2DOG (Z=13), from Thermus thermophilus HB8.

    Within the context of a 16S rRNA processing protein, this C-terminal domain might be involved in hydrophilic substrate (RNA) binding.  

    Ligand Summary





    No references found.

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