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The Open Protein Structure Annotation Network
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1z9t

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of Hypothetical UPF0124 protein yfiH (np_417084.1) from Escherichia coli K12 at 1.54 A resolution. To be published
    Site JCSG
    PDB Id 1z9t Target Id
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    Molecular Characteristics
    Source
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    Alias Ids
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    TPS1388,NP_417084.1
    Molecular Weight
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    Da.
    Residues
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    Isoelectric Point
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    Sequence
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      BLAST   FFAS

    Structure Determination
    Method XRAY
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    Chains 1
    Resolution (Å)
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    Rfree
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    Matthews' coefficent 2.47 Rfactor 0.142
    Waters
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    Solvent Content 49.84

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    Ligand Information
    Ligands
    Metals

    Jmol

     
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    Google Scholar output for 1z9t

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    Protein Summary

    The gene yfiH from Escherichia coli k12 encodes the NP_417084.1 protein, a putative multicopper polyphenol oxidase (laccase) PF02578 EC:1.10.3.2.  NP_417084 shows the conserved motif  H-71, 105-ADCLP-109, H-124 that includes the putative catalytic histidines capable of binding copper. Alternative name for this enzyme is urishiol oxidase. Its genome context indicates a possible functional link (score 0.96) with rluD, a ribosomal large subunit pseudouridine synthase D.  

    The 1z9t structure belongs to the class of alpha and beta (a+b) proteins and reveals a CNF1/YfiH-like putative cysteine hydrolases fold type SCOP64437.  According to DALI, 1z9t is structurally similar to the NMB0706 protein 1rv9 (Z=38), the protein CC_0490 (DUF152) from Caulobacter crescentus 1XFJ (Z=33), and the YLMD protein 1t8h (Z=32).

    Laccases act on phenols and similar molecules, performing a one-electron oxidations, which remain poorly defined. Example: 4 benzenediol + O(2) <=> 4 benzosemiquinone + 2 H(2)O.  It is proposed that laccases play a role in the formation of lignin by promoting the oxidative coupling of lignols, a family of naturally occurring phenols. The enzymatically active form of the enzyme can be a dimer or a trimer. 

    Ligand Summary



    References

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