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1vjz

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of Endoglucanase (TM1752) from Thermotoga maritima at 2.05 A resolution. To be published
    Site JCSG
    PDB Id 1vjz Target Id 359798
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1435,TM1752, 396397, 396393, 396396 Molecular Weight 39298.98 Da.
    Residues 329 Isoelectric Point 5.88
    Sequence mnntiprwrgfnlleafsikstgnfkeedflwmaqwdfnfvripmchllwsdrgnpfiiredffekidr vifwgekygihicislhrapgysvnkeveektnlwkdetaqeafihhwsfiarrykgissthlsfnlin eppfpdpqimsvedhnslikrtiteirkidperliiidglgygnipvddltientvqscrgyipfsvth ykaewvdskdfpvpewpngwhfgeywnrekllehyltwiklrqkgievfcgemgaynktphdvvlkwle dlleifktlnigfalwnfrgpfgildserkdveyeewyghkldrkmlellrky
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 2.05 Rfree 0.21244
    Matthews' coefficent 2.53 Rfactor 0.16804
    Waters 212 Solvent Content 51.06

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    Ligand Information
    Ligands
    Metals

    Jmol

     
    Google Scholar output for 1vjz
    1. The Buccaneer software for automated model building. 1. Tracing protein chains
    K Cowtan - Acta Crystallographica Section D: Biological , 2006 - scripts.iucr.org
     
    2. High-throughput protein production for X-ray crystallography and use of size exclusion chromatography to validate or refute computational biological unit predictions
    D McMullan, JM Canaves, K Quijano - Journal of structural and , 2005 - Springer
     
    3. Volsurf computational method applied to the prediction of stability of thermostable enzymes
    P Braiuca, A Buthe, C Ebert, P Linda - Biotechnology , 2007 - Wiley Online Library
     
    4. Tracing Determinants of Dual Substrate Specificity in Glycoside Hydrolase Family 5
    Z Chen, GD Friedland, JH Pereira, SA Reveco - Journal of Biological , 2012 - ASBMB
     
    5. THERMOSTABLE ENZYMES FOR THE HYDROLYSIS OF MANNAN-CONTAINING POLYSACCHARIDES
    Y Han, D Dodd, S Nair, RI Mackie - US Patent , 2011 - freepatentsonline.com
     

    Protein Summary

    The gene TM1752 from Thermotoga maritima encodes the enzyme endoglucanase PF00150 EC:3.2.1.4 COG4305.  Accepted name: cellulase; alternative names: celludextrinase, endo-1,4-beta-D-glucanase.  The enzyme belongs to the family of alpha and beta (a+b) proteins and reveals TIM beta/alpha-barrel fold type SCOP51350. The enzyme breaks down cellulose to beta-glucose. This type of cellulase is produced mainly by symbiotic bacteria in the ruminating chambers of herbivores.  The structures of several enzyme homologues from different organisms have been solved: 1CEN, Clostridium thermocellum.

    Ligand Summary



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