The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of Glucosamine-6-phosphate deaminase (TM0813) from Thermotoga maritima at 1.8 A resolution. To be published
    Site JCSG
    PDB Id 1j5x Target Id 282683
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1235,TM0813,, 282222 Molecular Weight 37369.23 Da.
    Residues 330 Isoelectric Point 5.75
    Sequence msktlkeitdqknelkkffenfvlnlekteifseiqknltdevlfvgcgssynlaltisyyfervlkir tkaipagevafqkipdleerglaflfsrtgnttevllandvlkkrnhrtigitieeesrlakesdlplv fpvreeaivmtksfsmillslmfladkiagnsterfselvgyspeffdiswkviekidlkehdhfvflg mseffgvslesalkciemsltfseaystleyrhgpkalvkkgtlvfmqkvsgmdeqekrlrkeleslga tvlevgeggdipvsndwksaflrtvpaqilgyqkaisrgispdkpphlektvvl
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.80 Rfree 0.231
    Matthews' coefficent 2.51 Rfactor 0.192
    Waters 294 Solvent Content 50.60

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    Ligand Information


    Google Scholar output for 1j5x
    1. Active site identification through geometry-based and sequence profile-based calculations: burial of catalytic clefts
    R Greaves, J Warwicker - Journal of molecular biology, 2005 - Elsevier
    2. High-throughput protein production for X-ray crystallography and use of size exclusion chromatography to validate or refute computational biological unit predictions
    D McMullan, JM Canaves, K Quijano - Journal of structural and , 2005 - Springer
    3. Computing protein structures from electron density maps: The missing fragment problem
    I Lotan, H Van Den Bedem, AM Deacon - Foundations of Robotics , 2005 - Springer
    4. Efficient recognition of protein fold at low sequence identity by conservative application of Psi_BLAST: validation
    FJ Stevens - Journal of Molecular Recognition, 2005 - Wiley Online Library
    5. Chronicles of the GTPase switch
    J Cherfils, M Zeghouf - Nature Chemical Biology, 2011 - nature.com
    6. Mutual information and variants for protein domain-domain contact prediction
    M Gomes, R Hamer, G Reinert - BMC Research , 2012 - biomedcentral.com

    Protein Summary

    The TM0813 gene from Thermotoga maritima encodes a glucosamine-6-phosphate deaminase (PF01380, COG0449, EC The TM0813 structure adopts a SIS (Sugar ISomerase) domain fold and shows strong similarity (main-chain rmsd of 1.8 Å over 291 residues with a sequence identity of 32%) with a glucosamine-6-phosphate deaminase from two other hyperthermophiles, Pyrococcus horikoshii (PDB ids: 2e5f, 2df8, 2dec) and Pyrococcus furiosus (2cb0) (Kim 2007). Similar results were obtained for a homolog from Listeria monocytogenes (PDB id: 2aml).


    [SIS domain fold family contains 3 children: mono-SIS, double-SIS and phosphoglucose isomerase (PGI) SIS. TM0813 is part of the double-SIS group along with 1wiw, 1tzb, 1tzc, 1x9i, 1x9h and others. Look at fold vs function variants]


    [Compare active site residues with 2cb0]

    Ligand Summary





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