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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of indole-3-glycerol phosphate synthase (TM0140) from Thermotoga maritima at 3.0 A resolution. To be published
    Site JCSG
    PDB Id 1j5t Target Id 282020
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1184,TM0140, 83901 Molecular Weight 26008.62 Da.
    Residues 230 Isoelectric Point 5.46
    Sequence mivqrrnhrflevlsgkervkiiaefkkaspsagdinadasledfirmydeladaisiltekhyfkgdp afvraarnltcrpilakdfyidtvqvklassvgadailiiariltaeqikeiyeaaeelgmdslvevhs redlekvfsvirpkiigintrdldtfeikknvlwellplvpddtvvvaesgikdprelkdlrgkvnavl vgtsimkaenprrfleemrawse
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 3.00 Rfree 0.297
    Matthews' coefficent 2.72 Rfactor 0.24
    Waters 6 Solvent Content 54.37

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    Ligand Information


    Google Scholar output for 1j5t
    1. PROuST: A comparison method of three-dimensional structures of proteins using indexing techniques
    M Comin, C Guerra, G Zanotti - Journal of Computational , 2004 - online.liebertpub.com
    2. The Crystal Structure of TrpD, a Metabolic Enzyme Essential for Lung Colonization by Mycobacterium tuberculosis, in Complex with its Substrate
    CE Lee, C Goodfellow, F Javid-Majd, EN Baker - Journal of molecular , 2006 - Elsevier
    3. Efficient recognition of protein fold at low sequence identity by conservative application of Psi_BLAST: validation
    FJ Stevens - Journal of Molecular Recognition, 2005 - Wiley Online Library
    4. Dimensionality reduction in computational demarcation of protein tertiary structures
    RR Joshi, PR Panigrahi, RN Patil - Journal of Molecular Modeling, 2011 - Springer
    5. Digital signal processing in predicting secondary structures of proteins
    D Mitra, M Smith - Innovations in Applied Artificial Intelligence, 2004 - Springer

    Protein Summary

    The TM0140 gene of Thermotoga maritima encodes an indole-3-glycerol phosphate synthase (IGPS) (EC, PF00218), an enzyme catalyzing the fourth step in the biosynthesis of tryptophan. The 1j5t structure comprises a single-domain (beta/alpha) 8 barrel (TIM barrel). With the exceptional of an additional N-terminal helix (missing from TM0140), the structure is essentially identical (main chain rmsd 1.6 Å over 207 residues) with the IGPS of Sulfolobus solfataricus, another hyperthermorphile, for which the active site and catalytic mechanism have been described (Hennig 2002) (Fig. 1).




    Figure 1. Ribbon diagram showing the superposition of the IGPS structures of T. maritima (PDB id: 1j5t, in blue) and S. solfataricus (PDB id: 1a53, in gray). The catalytically important residues are labelled for TM0140 and shown in ball-and-stick. The ligand (IGP) is shown in red.

    Ligand Summary





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