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The Open Protein Structure Annotation Network
PDB Keyword
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1j5t

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of indole-3-glycerol phosphate synthase (TM0140) from Thermotoga maritima at 3.0 A resolution. To be published
    Site JCSG
    PDB Id 1j5t Target Id 282020
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1184,TM0140, 83901 Molecular Weight 26008.62 Da.
    Residues 230 Isoelectric Point 5.46
    Sequence mivqrrnhrflevlsgkervkiiaefkkaspsagdinadasledfirmydeladaisiltekhyfkgdp afvraarnltcrpilakdfyidtvqvklassvgadailiiariltaeqikeiyeaaeelgmdslvevhs redlekvfsvirpkiigintrdldtfeikknvlwellplvpddtvvvaesgikdprelkdlrgkvnavl vgtsimkaenprrfleemrawse
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 3.00 Rfree 0.297
    Matthews' coefficent 2.72 Rfactor 0.24
    Waters 6 Solvent Content 54.37

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    Ligand Information
    Ligands
    Metals

    Jmol

     
    Google Scholar output for 1j5t
    1. PROuST: A comparison method of three-dimensional structures of proteins using indexing techniques
    M Comin, C Guerra, G Zanotti - Journal of Computational , 2004 - online.liebertpub.com
     
    2. The Crystal Structure of TrpD, a Metabolic Enzyme Essential for Lung Colonization by Mycobacterium tuberculosis, in Complex with its Substrate
    CE Lee, C Goodfellow, F Javid-Majd, EN Baker - Journal of molecular , 2006 - Elsevier
     
    3. Efficient recognition of protein fold at low sequence identity by conservative application of Psi_BLAST: validation
    FJ Stevens - Journal of Molecular Recognition, 2005 - Wiley Online Library
     
    4. Dimensionality reduction in computational demarcation of protein tertiary structures
    RR Joshi, PR Panigrahi, RN Patil - Journal of Molecular Modeling, 2011 - Springer
     
    5. Digital signal processing in predicting secondary structures of proteins
    D Mitra, M Smith - Innovations in Applied Artificial Intelligence, 2004 - Springer
     

    Protein Summary

    The TM0140 gene of Thermotoga maritima encodes an indole-3-glycerol phosphate synthase (IGPS) (EC 4.1.1.48, PF00218), an enzyme catalyzing the fourth step in the biosynthesis of tryptophan. The 1j5t structure comprises a single-domain (beta/alpha) 8 barrel (TIM barrel). With the exceptional of an additional N-terminal helix (missing from TM0140), the structure is essentially identical (main chain rmsd 1.6 Å over 207 residues) with the IGPS of Sulfolobus solfataricus, another hyperthermorphile, for which the active site and catalytic mechanism have been described (Hennig 2002) (Fig. 1).

     

    1j5t_1a53_IGP.png

     

    Figure 1. Ribbon diagram showing the superposition of the IGPS structures of T. maritima (PDB id: 1j5t, in blue) and S. solfataricus (PDB id: 1a53, in gray). The catalytically important residues are labelled for TM0140 and shown in ball-and-stick. The ligand (IGP) is shown in red.

    Ligand Summary



    References

    Reviews

    References

     

    No references found.

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    214.02 kB01:55, 10 Jan 2009tinabActions
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